Specific Aim 2 To Determine If Gleevec Is Effective In

Specific Aim 2: To determine if Gleevec is effective in inhibiting in vivo cell proliferation in TNBC cells with overexpressed Arg A recombinant plasmid containing a viral promoter driving Arg expression will be inserted into TNBC cells. Another empty recombinant plasmid will be inserted into different TNBC cells. Another plasmid containing a promoter driving Arg expression will be inserted into another group of TNBC cells. Soon after, an immunoblot will be run using these three different TNBC groups’ cells to ensure the proper transfer of plasmid. The proteins we will test for include Arg, Abl, actin, and Ras-MAPK. Actin is again serving as a positive control, while the expression of Ras-MAPK proteins can act as a quantitative†¦show more content†¦Abl is known to mutate its kinase domain so that Gleevec cannot effectively inhibit it and cellular proliferation (Lovera et al., 2015). A western blot will be run to determine the extent this mutation will affect our exper iment at the beginning and 4 months into our experiment. Some tumors from all three cell lines will have their cells lysed and run through the gel, which will test for both Abl and Abl-Gleevec compound, as well as a major substrate for Arg-Abl, ArgBP2. Results- The western blot containing the three different cell lines expressed an equal amount of actin expressed in all three lanes. This served as a control to ensure the plasmids were inserted correctly and there was no interference of any other gene. The lane with cells from mice group 1 showed darker bands on lines corresponding to the proteins Arg as opposed to the lane with mice group 2 cells. The bands corresponding to the protein Abl appeared to be the same on both lanes, demonstrating that Abl was not being accidentally overexpressed as well. These pieces of information elucidated to us that the Arg gene was being successfully overexpressed in line 1. The bands corresponding to Ras-MAPK were darker for mice group 1 than for group 2, demonstrating that this signal transduction pathway for cell proliferation has been downregulated with the overexpression Arg.Show MoreRelatedBrand Building Blocks96400 Words   |  386 Pagescan inhibit the brand builder. To be able to develop effectiv e brand strategies, it is useful to understand these pressures and barriers Different factors that make it difficult to build brands are shown in the figure above. The first, pressure to compete on price, directly affects the motivation to build brands. The second reason, the proliferation of competitors, reduces the positioning options available and makes implementation less effective. The third and fourth reasons, the fragmentation inRead MorePharmaceutical Price Controls in the Oecd Countries47662 Words   |  191 Pageselectronic reprint from the National Technical Information Service, 5285 Port Royal Road, Springfield, VA 22161; www.ntis.gov. ii U.S. Department of Commerce, International Trade Administration Contents Executive Summary vii 1 Introduction 1 2 Drug Price Regulations in Selected OECD Countries—An Overview of the Issues 3 3 Price And Revenue Effects 10 4 Impact of Deregulating Prices on Research and Development, Innovation, and Consumers 25 Appendix A: Technical Methodology 35 Appendix B: DrugRead MoreAnnotated Bibliography: Plagiarism39529 Words   |  158 Pageslike BJET. It is essential that we distinguish between the work that has been carried out by others and the work and the writing that is due to our original efforts. Most research projects will start with a comprehensive study of the literature to determine what has been found in the past, and it is that foundation that will inform the subsequent work. It is quite likely that, in order to make the argument for the paper, you will need to quote from the work of others. However, you must make it quite